Table 2.
Primers Used for Real-Time Reverse Transcriptase–Polymerase Chain Reaction
| Gene | Accession no. | Primer sequence (5′-3′) | Size (bp) | Annealing (°C) | Recognition tissue(s) |
|---|---|---|---|---|---|
| OCT4 | NM_013633 | F:GTTCTCTTTGGAAAGGTTC | 146 | 55 | ESC |
| R:GCATATCTCCTGAAGGTTCT | |||||
| Nanog | NM_028016 | F:TGATTTGGTTGGTGTCTTG | 175 | 55 | ESC |
| R:TGTGATGGCGAGGGAAG | |||||
| Sox2 | NM_011443 | F:CTGGAGAAGGGGAGAGATTTT | 157 | 55 | ESC |
| R:CGTTAATTTGGATGGGATTGG | |||||
| ALP | NM_007433 | F:GTGTTGTGGTATTGCAGCTTCT | 248 | 58 | ESC |
| R:CATCCCATCTCCCATGAGGAT | |||||
| THY1 | NM_009382 | F:TCAAGTGTGGCGGCATAAG | 77 | 58 | T-cells/MSC |
| R:GAGGAGGGAGAGGGAAAGC | |||||
| Sox1 | NM_009233 | F:GCTACATGAGCGCGTCGCCT | 160 | 58 | Neuroectoderm |
| R:AGAGATCCGAGGGCGCCCAG | |||||
| gFAP | NM_010277 | F:AGGGGCCTCGGTCCTAGT | 137 | 60 | Glial/Asterocyte |
| R:CGCCCGTGTCTCCTTGAAG | |||||
| TH | NM_009377 | F:GCCACAGCCCAAGGGCTTCA | 101 | 58 | Neuron/Dopaminergic |
| R:TGAGACTCTGCCGCCGTCCA | |||||
| Nestin | NM_016701 | F:AGCAGGGTCTACAGAGTCAG | 100 | 58 | Neuronal precursors |
| R:GTCCTGTATGTAGCCACTTCC | |||||
| GSC | NM_010351 | F:GCTGGCCAGGAAGGTGCACC | 148 | 58 | Mesendodrm/endoderm |
| R:CGGCGAGGCTTTTGAGGACGT | |||||
| Albumin | NM_009654 | F:CAGGATTGCAGACAGATAGTC | 265 | 60 | Hepatocyte |
| R:GCTACGGCACAGTGCTTG | |||||
| TTR | NM_013697 | F:GGCTGAGTCTCTCAATTC | 223 | 58 | Hepatocyte |
| R:CTCACCACAGATGAGAAG | |||||
| TAT | NM_146214 | F:ACCTTCAATCCCATCCGA | 205 | 55 | Hepatocyte |
| R:TCCCGACTGGATAGGTAG | |||||
| Foxa2 | NM_010446 | F:CCAGCGAGTTAAAGTATGC | 150 | 55 | Definitive endoderm |
| R:TGTGTTCATGCCATTCATC | |||||
| HNF4α | NM_008261 | F:ACA CGT CCC CAT CTG AAG | 269 | 55 | Hepatocyte |
| R:CTT CCT TCT TCA TGC CAG | |||||
| Brachuruy | NM_009309 | F:CCCTGCACATTACACACCAC | 154 | 58 | Mesendoderm/Mesoderm |
| R:AGGTGTCCACGAGGCTATGA | |||||
| MyoD2 | NM_010866 | F:CTGATGGCATGATGGATTAC | 120 | 58 | Muscle |
| R:GACACAGCCGCACTCTTC | |||||
| Myf5 | NM_008656 | F:CGCTGGTCGCTGGAGAG | 292 | 60 | Muscle |
| R:GAGGGAACAGGTGGAGAACTA | |||||
| α-MHC | NM_010856 | F:CCAGATTATCCAGGCTAACC | 151 | 60 | Cardiomyocyte |
| R:CCAGAAGGTAGGTCTCTATGT | |||||
| β-MHC | NM_080728 | F:GCTGTTTCCTTACTTGCTACC | 116 | 60 | Cardiomyocyte |
| R:GGATTCTCAAACGTGTCTAGT | |||||
| Troponin-T | NM_011619 | F:CGAGCAGCAGCGTATTC | 110 | 58 | Cardiomyocyte |
| R:CCTCATCCTCAGCCTTCC | |||||
| Adipsin | NM_013459 | F:ATGGTATGATGTGCAGAGTGT | 448 | 60 | Adipocyte |
| R:CACACATCATGTTAATGGTGAC | |||||
| Osteocalcin | NM_007541 | F:GACCATCTTTCTGCTCACTCTG | 230 | 55 | Adipocyte |
| R:GTGATACCATAGATGCGTTTGT | |||||
| Osteopontin | NM_009263 | F:CAGTGATTTGCTTTTGCCTGTT | 362 | 60 | Adipocyte |
| R:GGTCTCATCAGACTCATCCGA | |||||
| VegFR2 | NM_010612 | F:GCCATCAACAAAGCGGGACGA | 200 | 60 | Endothelium |
| R:GATTCGCCCATGTGGACCGAT | |||||
| VWF | NM_011708 | F:GACAGACGCCATCTCCAG | 175 | 58 | Endotelium |
| R:ATGTCTCATCTTGCTTCAGG | |||||
| Dazl | NM_010021 | F:ATCAGCAACCACAAGTCAAG | 187 | 55 | Germ cells |
| R:CAAATCCATAGCCCTTCG | |||||
| Sycp3 | NM_011517 | F:AAAGCATTCTGGGAAATCTG | 187 | 58 | Germ cells |
| R:GTACTTCACCTCCAACATCTTC | |||||
| Ddx4 | NM_010029 | F:CGGAGAGGAACCTGAAGC | 160 | 58 | Germ cells |
| R:CGC CAATATCTGATGAAGC | |||||
| HPRT | NM_013556 | F:GGCCAGACTTTGTTGGATTTG | 144 | 55-60 | Internal standard |
| R:TGCGCTCATCTTAGGCTTTGT |
ESC, embryonic stem cell; MSC, mesenchymal stem cell.