Table 2.
Antibody Response of Rhesus Macaques to rEZ Vectored gp350 or F
| Inoculation | Prior MV Vaccination a | MV Neutralizing Ab (mean log2 ± SD Titer) | RSV or EBV ELISA Ab (Mean log2 ± SD Titer) | RSV or EBV Neutralizing Ab (Mean log2 ± SD Titer) c | ||||
|---|---|---|---|---|---|---|---|---|
| Day 0 | Dose 1 b | Dose 2 b | Dose 1 | Dose 2 | Dose 1 | Dose 2 | ||
| gp350 | + | 4.3 ± 1.2 | 5.7 ± 0.5 | 7.3 ± 0.6 | < 3.3 | < 3.3 | < 3.3 | < 3.3 |
| gp350 | - | < 3.3 | 5.4 ± 1.1 | 7.3 ± 0.1 | < 3.3 | < 3.3 | < 3.3 | < 3.3 |
| sF3 | + | 4.2 ± 0.7 | 5.2 ± 1.5 | 7.5 ± 1.2 | 9.3 ± 1.9 | 11.8 ± 1.0 | 3.3 ± 0.5 | 5.0 ± 0.8 |
| sF3 | - | < 3.3 | 5.9 ± 1.1 | 7.9 ± 1.1 | 8.8 ± 1.8 | 9.5 ± 2.1 | 3.3 ± 0.5 | 4.5 ± 1.7 |
a
Previously vaccinated with Measles vaccine.
b
Serum samples were measured 4 weeks post dose 1 and 2 weeks post dose 2.
c
RSV antibody titer is defined as log2 reciprocal dilution of serum that reduced plaques on HEp-2 cells by 50%; neutralization assay was performed with 10% guinea pig complement.
Titers of prevaccinated animals were under the limit of detection in all the assays.