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. 2012 Feb 24;442(Pt 3):563–572. doi: 10.1042/BJ20111360

Figure 1. Crammer dimerization as a function of pH.

Figure 1

(A) SEC is used to monitor the dimerization of crammer as a function of pH. Blue Dextran 2000 as the internal standard is eluted at the void volume. Molecular mass standards: 1, chymotrypsinogen A (25 kDa) and 2, RNase A (13.7 kDa). (B) 13% (w/v) Tricine-SDS/PAGE. Lane 1 contains molecular size markers (M). Lanes labelled monomer and dimer are HPLC-purified proteins, and the proteins contained in last two lanes are as labelled in the Figure. β-ME, 2-mercaptoethanol. (C) The results from SEC and Tricine-SDS/PAGE suggest that C72S at neutral pH exists in a monomeric form. (D) Crammer extracted from Drosophila heads is monomeric in the absence of 2-mercaptoethanol. The control lane labelled ‘crammer’ contains the recombinant protein, which exists as both monomer and dimer as visualized by the antibody against crammer. In (B) and (D) the molecular mass is given in kDa on the left-hand side. AU, arbitrary unit.