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. 2012 Feb 24;442(Pt 3):563–572. doi: 10.1042/BJ20111360

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Far-UV CD of crammer at various solution pH values and temperature. WT, wild-type. Inset: thermal denaturation of crammer and C72S monitored by CD at 208 nm. (B) The spectral properties of C72S as a function of pH monitored by far-UV CD and intrinsic fluorescence spectroscopy and ANS binding.