Figure 1.  Alternative splicing changes induced by an expanded CUG repeat-containing RNA can be rescued in cultured cells by repression of CELF activity.  Expression of an RNA containing 960 CUG repeats promotes exon inclusion of the R35C chicken cTNT minigene reporter in differentiated C2C12 myotubes.  This effect is rescued by additional co-expression with NLSCELF∆, a dominant negative CELF protein.  (A) A representative RT-PCR gel showing R35C alternative exon included and exon skipped products.  (B) Quantitation of the extent of R35C exon inclusion and exon skipping in multiple independent transfection experiments (n = 4 to 6 samples per group).