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. 2012 Feb 24;442(Pt 3):539–550. doi: 10.1042/BJ20111678

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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B13 cells were fixed and stained 3, 6, 9, 12 days after Ad-PNM transduction. Cells were then co-stained for insulin (red) and Pdx1 (green) (A), insulin (red) and Ngn3 (green) (B), insulin (red) and MafA (green) (C). (D) Percentage of insulin-positive cells out of the population of PNM-positive cells. Data are means±S.E.M. (n=3). (E) C-peptide (green), insulin (red) and DAPI (blue) at day 12. (F) Amylase (green), insulin (red) and DAPI (blue) at day 6. Scale bars=100 μm.