Figure 7. Location of autophosphorylation sites within the domain structure of eEF2K.

The layout of the eEF2K polypeptide is depicted schematically (and only roughly to scale). The major sites of autophosphorylation identified in the present study are indicated (black arrows), with minor ones shown by grey arrows. The dotted arrow indicates that phosphorylated Thr³⁴⁸ may dock with the phosphate (P-) pocket in the C-terminal lobe of the catalytic domain to promote eEF2K activity, similar to Thr⁸²⁵ in MHCKA [25]. See the Discussion for further information.