Table 2. Sequences of the WX(F/W)(S/T)-like sequence in GCKIII and GCKVI subgroups in H. sapiens and mouse.
The WX(F/W)(S/T)-like sequence corresponds to site D in STRADα [4]. In Xenopus laevis MST3 (GenBank® acession number NP_001085728), the sequence is W323NFTRR328, and in Danio rerio MST3 (GenBank® acession number NP_998642), it is W315IFTIR320. It is also conserved in MST3 of many other vertebrates. For the GCKVI subgroup, the STRADα site D-like ‘WEW’ sequence has been extended on either side to show the acidic nature of the region.
| GCK subgroup | GenBank® accession number (H. sapiens), with residue numbers of the WX(F/W)(S/T)-like sequence in parentheses | GenBank® accession number (mouse), with residue numbers of the WX(F/W)(S/T)-like sequence in parentheses | WX(F/W)(S/T)-like sequence |
|---|---|---|---|
| GCKIII subgroup | |||
| MST3 (Stk24) | NP_001027467 (325–330) | NP_663440 (325–330) | WIF–TIR |
| YSK1/SOK1 (Stk25) | NP_006365 (319–326) | NP_067512 (319–326) | WTFPPTIR |
| MST4/MASK | NP_057626 (324–330) | NP_598490 (324–330) | WSFT-TVR |
| GCKVI subgroup | |||
| SPAK/PASK (Stk39) | NP_037365 (378–395) | NP_058562 (390–407) | EDGDWEWSDDEMDEKSEE |
| OXSR1/OSR/KIAA1101 | NP_005100 (332–349) | NP_598746 (332–349) | EDGGWEWSDDEFDEESEE |