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. 2011 Sep 21;109(8):2772–2777. doi: 10.1073/pnas.1017626108

Fig. 2.

Fig. 2.

EpCAM+CD10− luminal and CD10+ basal/ME sorted populations contain cells with distinct functional characteristics. (A and B) Unsorted or sorted fractions of cells were plated in adherent conditions (A) or nonadherent conditions (B) and allowed to grow for 7–10 d. Graphed data represent average fold ± SE from independently sorted patient samples (n = 4–5). P values were calculated by Student's t test. (A Left) Representative image of suspension colonies (arrows) and adherent colonies. (Bar: 200 μm.) (Right) Representative images of crystal violet-stained colonies. (B) Immunofluorescence staining for CK8/18 (red) and CK14 (green) in spheres formed in nonadherent conditions. (Bar: 100 μm.) (C) Outgrowth from unsorted cells or sorted fractions plated on collagen I gels. (Upper) Representative images of the three structures formed. (Bar: 100 μm.) Graphed data represent average number ± SE from independently sorted patient samples (n = 3). P values were calculated by Student's t test. (D) Immunohistochemistry for luminal (CK19 and ERα) and basal/ME (CK14 and αSMA) differentiation markers in bilayered structures formed in vivo in the HIM model by sorted fractions. (Bar: 25 μm.)