Fig. 4.

Glucose tolerance tests after ablation of AgRP or MCH neurons. (A and B) Obese Lep^ob/ob and lean Lep^ob/+ mice carrying the Agrp^DTR allele were injected with either saline (SAL) or DT at 2 wk before glucose tolerance testing. Both males (A) and females (B) were tested. Error bars represent mean ± SEM (n = 5–11 per group). Area under the curve (AUC) measurements revealed significantly lower values in mice that received DT compared with obese Lep^ob/ob controls (P < 0.002, one-way ANOVA with Dunnett's posttest), but not significantly greater values than those in leptin-replete, lean mice. (C and D) Glucose tolerance tests were performed as in A and B, but with the Pmch^DTR allele instead of the Agrp^DTR allele present. Error bars represent mean ± SEM (n = 3–8 per group). P < 0.004 for AUC analyses.