Fig. 1.
Proteomic assessment of STK33 autophosphorylation. Sites on STK33 determined to be autophosphorylated are shown on the left. Increasing concentrations of the three fasudil analogs shown were added to an in vitro phosphorylation reaction in which the only substrate present was STK33 itself. The heatmap is colored according to how the level of phosphorylation at a given site changed in response to addition of compound at the specified concentration vs. DMSO control. Some compound concentrations were found to increase phosphorylations at given sites, leading to values > 100%. 1ambiguously localized phosphorylation within the span of bracketed amino acids. 2ambiguously localized double phosphorylation within the span of bracketed amino acids.