Fig. 4.

MST4 and ERK kinase activity was used as surrogate biomarker and negative control for BRD8899, respectively. (A and B.) NOMO-1 cells were treated with the indicated concentrations of staurosporine, the farnesyl transferase inhibitor L744 (as positive controls) or the fasudil analog BRD8899 for 24 h. Samples were lysed and subjected to immunoblotting for phosphorylated and total levels of Ezrin (T576) and total MST4 (A) or phosphorylated and total levels of ERK (B). Quantifications for each lane were generated using ImageJ software. The normalized values to total protein levels are presented next to the blots.