Fig. 1.

TLE1 binds to chromatin at ER binding events and is not influenced by estrogen. (A) TLE1 ChIP after vehicle (white bars) or estrogen (black bars) treatment followed by real-time PCR of ER binding sites. TLE1 binds to a subset of regions tested independently of ligand. The data are the average of three replicate experiments ± Std Dev. (B) siRNA to TLE1 (or control siRNA) was transfected into cells and total protein was immunoblotted. The uncropped figure is in Fig. S1. C. siControl or siTLE1 transfected MCF-7 cells were treated with estrogen for 24 h and %S phase was determined by PI staining and flow cytometry. The %S phase was decreased when TLE1 was silenced. The data are the average of three independent replicates ± Std Dev and example histograms are shown in Fig. S1.