Fig. 3.

TLE1 dependent ER binding events are less likely to be cobound by FoxA1. (A) Heat map showing ER binding signal intensity for each individual ER ChIP-seq replicate following transfection of siTLE1 or control siRNA. The heat map represents binding signal intensity with binding events ranked from the strongest to the weakest binding events. Each line represents an individual binding event, and the data are shown in a window of ± 5 kb around the center of the binding event. Also shown is the FoxA1 binding signal intensity in each category at the corresponding region and the fraction of each category (TLE1-independent or TLE1-dependent ER binding events) that overlap with a FoxA1 binding event. (B) Hormone-deprived MCF-7 cells were transfected with siRNA to TLE1 (or control siRNA) or FoxA1. ChIP of the reciprocal factor was performed followed by real-time PCR of known ER binding events. FoxA1 binding was not influenced by silencing of TLE1, but TLE1 binding was decreased when FoxA1 was silenced. N/S denotes not significant and * denotes p < 0.05. (C) Changes in mRNA levels of FoxA1 or TLE1 when the other gene was silenced by siRNA.