Fig. 2.
[Mg2+]-dependence of TL-R RNA docking at 20 ± 1 °C: (A) kdock, kundock and (B) Kdock = kdock/kundock described by a four-state model allowing for [Mg2+]-dependent and independent docking pathways (U = undocked, D = docked) and Mg2+ binding is not resolved by FRET. From this model, kdock = {k1(Kd)n + k2[Mg2+]n}/{(Kd)n + [Mg2+)} and 
. Fits of the kdock and kundock titrations with the detailed balance constraint that 
, yield n = 1.8 ± 0.2, k1 = 12.6 ± 0.9 s-1, k2 = 156 ± 23 s-1, k-1 = 8.6 ± 0.7 s-1, k-2 = 5.4 ± 0.2 s-1, Kd = 1.3 ± 0.3 mM, and 
.