Fig. 3.

XY-specific expression of amhy mRNA during embryogenesis and gonadal sex differentiation. (A and B) Temporal expression of amhy (1,699 bp) and amha (1,716 bp) mRNAs by RT-PCR in (A) embryos (daf: days after fertilization) and in (B) XX and XY larval trunks (wah: weeks after hatching). The β-actin (457-bp) gene was used as endogenous control for all samples. T: testis; O: ovary. (C) Localization of amhy mRNA (Right) in gonadal sections of XX and XY larvae at 3 wk after hatching (in situ hybridization with an antisense probe). (Left) H&E-stained sections of the same individuals. (Scale bars, 10 μm.)