Fig. 4.

Feminization in amhy knockdown XY fish. (A) Relative expression of foxl2 and cyp19a1a mRNAs in morpholino-injected fish at 4 weeks after hatching (wah). foxl2 and cyp19a1a expression was normalized by the respective β-actin values. The three XY individuals in the antisense morpholino group with female-like, high foxl2 values also show high values for cyp19a1a. (B) Histological appearance of the gonads in mispaired morpholino-injected XX control fish (XX-ctr), antisense morpholino-injected XY fish (XY-mo), and mispaired morpholino-injected XY fish (XY-ctr) at about 12 wah. OC: ovarian cavity. (Scale bars, 20 μm.)