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. 2012 Jan 30;109(8):E471–E480. doi: 10.1073/pnas.1115495109

Fig. 4.

Fig. 4.

Time course and effect of Rabenosyn-5 depletion on levels of endocytic proteins. Cells were transfected with scrambled (Sc) or Rabenosyn-5–directed (Si) oligonucleotides and at the time points indicated (A) or at 72 h (B) were analyzed by Western blotting for the proteins indicated. (C) Cells were transfected with scrambled (Sc), two different EEA1-directed (SiEEA1 a and,b), or Rabenosyn-5–directed (Si Rbsn) oligonucleotides and at 72 h were analyzed by Western blotting for the proteins indicated. (D) (Left) Representative TIRF (Upper) and epifluorescence (EPI) (Lower) images of endogenous TfR in cells transfected with scrambled (Sc) or Rabenosyn-5–directed (Si) oligonucleotides for 72 h. Epifluorescence images are autoscaled for illustration purposes to reveal the region of maximal TfR concentration. (Right) Quantification of the mean intensity of endogenous TfR in TIRF and EPI images.