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. 2012 Feb 6;109(8):3119–3124. doi: 10.1073/pnas.1109251109

Fig. 1.

Fig. 1.

The ChAT::Cre transgene is specifically expressed by Lhx7+ forebrain cholinergic neurons. (A–D) Ventral forebrain sections from E13.5 (A) or E14.5 (B–D) ChAT::Cre;Lhx7+/LacZ;R26ReYFP embryos immunostained for Cre, YFP, and β-gal (A and B) or YFP (C) or processed for ISH using a ChAT-specific riboprobe (D). Arrows in A and B indicate Cre+YFP− cells, and arrowheads in B indicate Cre+YFP+ cells. All Cre+ cells express β-gal. In the serial sections of ventral forebrain shown in C and D, the distribution of YFP signal is similar to that of ChAT mRNA. (E and F) Sections from the MGE-LGE boundary sections of E14.5 ChAT::Cre;Lhx7+/LacZ;R26ReYFP embryos triple-immunostained for YFP, Lhx6, and β-gal (E) or YFP, Isl1, and β-gal (F). Lhx6-expressing cells (arrowheads) are negative for YFP, and YFP+ cells (arrows) do not express Lhx6 (E). In contrast, YFP is detected in β-gal+ neurons expressing Isl1 (F). (G) Adult striatum sections immunostained for ChAT and YFP. YFP is expressed in virtually all cholinergic (ChAT+) neurons (arrows); some noncholinergic cells also express YFP (arrowheads). [Scale bars: 50 μm (A, B, E, and F); 100 μm (C and D).]