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. 2012 Feb 8;109(8):2989–2994. doi: 10.1073/pnas.1116418109

Fig. 1.

Fig. 1.

A subset of lymphoma cell lines exhibits elevated H3K27me3 levels. (A) Global H3K27me3 levels (normalized to total H3) were determined for 111 cancer cell lines from seven different cancer types using H3K27me3 and total H3 ELISAs. Lymphoma cell lines harboring heterozygous Y641 mutations are indicated by black tick marks below the graph. MM, multiple myeloma. (B) Western blot analysis was performed with antibodies specific for H3K27me3, H3K27me2, H3K27me1, total histone H3, EZH2, and actin using protein lysates from a panel of lymphoma cell lines. Actin serves as a loading control. EZH2 mutation status as determined from full-length Sanger sequencing is indicated.