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. Author manuscript; available in PMC: 2012 Feb 27.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2007 May 11;293(2):L480–L490. doi: 10.1152/ajplung.00345.2006

Fig. 2.

Fig. 2

Confirmation of DNA binding activity of TS-regulated transcriptional factors using EMSA. Cells were treated with TS and nuclear proteins were extracted and incubated with biotin-labeled probes corresponding to different TF binding sequences for EMSA as described in MATERIALS AND METHODS. A: GATA. B: NF-κB. C: PAX5. D: Smad 3/4. E: ISRF. F: ICSBP. Lane 1 of each panel indicates free probes. DNA binding activity is represented without (lane 2) and with (lane 3) TS exposure in each panel. Lane 4 of each panel represents binding activity after TS exposure but with 20× excess of cold probes added to the incubation. These results are representative of 3 independent experiments. SMK, smoke.