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. Author manuscript; available in PMC: 2012 Feb 27.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2007 May 11;293(2):L480–L490. doi: 10.1152/ajplung.00345.2006

Fig. 3.

Fig. 3

DNA binding activity of several TS-regulated TFs are susceptible to ERK 1/2 inhibition. A549 cells in a T25 flask were treated with TS as previously described and pretreated without inhibitor (A), with 10 μM U0126 (B), and with 20 μM PD98059 (C) for 2 h. The comparable signal intensity and contrast of the membranes were adjusted according to the alignment spots along the right and bottom edges, respectively. The TS-regulated TFs putatively mediated by ERK 1/2 were outlined with black boxes as indicated in D. These results are representative of 3 independent experiments.