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. Author manuscript; available in PMC: 2012 Feb 27.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2007 May 11;293(2):L480–L490. doi: 10.1152/ajplung.00345.2006

Fig. 7.

Fig. 7

Phosphorylation of ERK 1- and ERK 2-mediated DNA binding activity of NF-κB. A: serum-starved A549 cells in a T25 flasks pretreated without or with 20 μM PD98059 or 10 μM U0126 for 2 h and then exposed to 3 ml of freshly generated smoke for 1.5 h. B: stably transfected control and dominant negative cells (A549-pCEPT4, A549-dnERK 1, and A549-dnERK 2) were treated without or with TS exposure of 3 ml of freshly generated smoke for 1.5 h as described in MATERIALS AND METHODS. Extracted nuclear proteins were incubated with biotin-labeled probes corresponding to NF-κB binding sequences for EMSA.