Figure 3.

Effects of the treatments with OVA on the frequency of CD103⁺ cells in the small intestine. Freshly isolated IELs from BALB/c (a) and DO11.10 (b) mice were gated for CD3⁺/CD4⁺, CD3⁺/CD8α ⁺, and CD3⁺/CD8β ⁺ cells and analyzed for expression of CD103 by flow cytometry. Blank histograms indicate isotype control staining. CD3⁺/CD8β ⁺ subset illustrated at the right column is part of the CD3⁺/CD8α ⁺ population. (c) Data represent mean ± SEM (N = 5) in three independent experiments. Frequency of CD4⁺ cells were significantly more elevated in IELs isolated from mice BALB/c and DO11.10 treated with OVA by oral + ip routes. The expression of CD103 was markedly augmented in all subpopulations of IELs of OVA-treated BALB/c mice and was reduced in DO11.10 immunized by ip route.