Figure 3.

Degeneration of Bergmann glial fibers in APC-CKO mice during the late postnatal period. Representative images of the cerebellar cortices (A–F) and their magnified views (A′–F′) stained by either Nestin or GFAP in control (Ctrl) and CKO mice at different postnatal ages. At P3, Bergmann glia are positive for an embryonic radial glia marker Nestin and their radial fibers are indistinguishable between Ctrl (A) and CKO mice (B). At P10, the radial fibers are positive for GFAP and the density of GFAP⁺ fibers is only slightly decreased in CKO mice (D) as compared to that in Ctrl(C). At P21, the radial fibers are markedly decreased, whereas stellate-shaped GFAP⁺ cells are present in the MOL of APC-CKO mice (F). In contrast, the distribution and morphology (inset) of GFAP-positive astrocytes in the granule cell layer (G, H), the cerebellar white matter (I, J), and the pons (K, L) are indistinguishable between Ctrl and CKO mice at P21. Scale Bar: 30 μm. P, postnatal day; PCL, Purkinje cell layer; MOL, molecular layer; GCL, granule cell layer; WM, cerebellar white matter.