Figure 1.

Identification of PHB by 2-D gel electrophoresis and mass spectrometry. A, Mitochondrial proteins from FN- and DN-stimulated rat brain were separated on 2-D gels, and protein spots were visualized by Ruby Red staining. The open arrow indicates the spot that corresponds to PHB with a probability of 100%. The closed arrow indicates a spot that corresponds to annexin V binding protein with 60% probability. B, Western blot of mitochondrial proteins from FN- and DN-stimulated rat brain confirming that PHB is upregulated in mitochondria of brains preconditioned with FN stimulation. The mitochondrial marker cytochrome c oxidase (COX IV) was used to assure equal gel loading. Each lane contains 10 μg of mitochondria. C, Quantification of band intensity in B. *p < 0.05; n = 7/group. Error bars indicate SEM.