Figure 5.

Functional annotation of uncharacterized query protein ne0167 (PDB/Chain ID: 3k6c.H) from a Structural genomics project. (a) Putative binding site is shown as spacefill model and is colored orange. The rest of the query protein is shown as a cartoon model. (b) Among the top similar proteins are ferritin heavy chain (2cih.A), chloroplastic ferritin 4 (3a68.B), and various bacterioferritins (2fkz.G, 3gvy.A, 1jgc.A, and 2vzb.B). The top ranked proteins (1jm0.C and 1y4dt.B) are de novo designed protein structures that bind Fe²⁺ ions. (c) Superimposition of putative binding site in query protein (3k6c.H) and known Fe²⁺ ion binding site in bacterioferritin (2kfz.G). The two Fe²⁺ ions co-crystallized with bacterioferritin are brown spheres. (d) The detailed pairwise alignment of query and bacterioferritin proteins.