Table 2.
Serum analytes significantly elevated in COPD
| Analytea | Medianb | Fold (COPD/control)c | FDRd | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| BS | T54 | Ctr1 | Ctr2 | BS/Ctr1 | T54/Ctr1 | BS/Ctr2 | T54/Ctr2 | |||
| COPD > Control | ||||||||||
| CD40 Ligand | ng/mL | 2.50 | 0.96 | 0.01 | 0.01 | 250 | 95.9 | 250 | 95.9 | < 10-8 |
| EGF | pg/mL | 699.49 | 230.99 | 14.84 | 3.70 | 47.1 | 15.6 | 189 | 62.4 | < 10-8 |
| BDNF | ng/mL | 28.50 | 24.80 | 0.52 | 0.56 | 55.0 | 47.9 | 50.5 | 44.0 | < 10-8 |
| RANTES | ng/mL | 18.65 | 15.90 | 1.28 | 1.22 | 14.5 | 12.4 | 15.2 | 13.0 | < 10-8 |
| Myeloperoxidase | ng/mL | 2144.71 | 686.50 | 119.47 | 87.50 | 18.0 | 5.7 | 24.5 | 7.8 | < 10-8 |
| Eotaxin | pg/mL | 232.50 | 162.50 | 20.50 | 20.50 | 11.3 | 7.9 | 11.3 | 7.9 | < 10-8 |
| EN-RAGE | ng/mL | 254.97 | 35.60 | 13.40 | 14.50 | 19.0 | 2.7 | 17.6 | 2.5 | < 10-8 |
| Ferritin | ng/mL | 166.00 | 113.00 | 18.95 | 28.00 | 8.8 | 6.0 | 5.9 | 4.0 | < 10-8 |
| IL-1RA | pg/mL | 360.50 | 111.50 | 31.82 | 38.00 | 11.3 | 3.5 | 9.5 | 2.9 | < 10-8 |
| ENA-78 | ng/mL | 2.07 | 1.68 | 0.32 | 0.35 | 6.5 | 5.2 | 5.9 | 4.8 | < 10-8 |
| PAI-1 | ng/mL | 199.00 | 183.50 | 26.05 | 54.00 | 7.6 | 7.0 | 3.7 | 3.4 | < 10-8 |
| MCP-1 | pg/mL | 558.00 | 483.00 | 170.00 | 100.00 | 3.3 | 2.8 | 5.6 | 4.8 | < 10-8 |
| MIP-1beta | pg/mL | 341.49 | 259.50 | 99.00 | 87.50 | 3.4 | 2.6 | 3.9 | 3.0 | < 10-8 |
| Thrombopoietin | ng/mL | 4.92 | 4.70 | 1.62 | 1.60 | 3.0 | 2.9 | 3.1 | 2.9 | < 10-8 |
| TIMP-1 | ng/mL | 211.00 | 192.00 | 70.44 | 67.00 | 3.0 | 2.7 | 3.1 | 2.9 | < 10-8 |
| IL-16 | pg/mL | 1174.99 | 440.50 | 233.49 | 273.50 | 5.0 | 1.9 | 4.3 | 1.6 | < 10-8 |
| VEGF | pg/mL | 1275.00 | 933.00 | 447.00 | 383.50 | 2.9 | 2.1 | 3.3 | 2.4 | < 10-8 |
| Cancer antigen 19-9 | U/mL | 7.19 | 5.94 | 2.11 | 3.30 | 3.4 | 2.8 | 2.2 | 1.8 | 3.7 × 10-7 - 4.5 × 10-4 |
| CD40 | ng/mL | 1.84 | 1.06 | 0.61 | 0.59 | 3.0 | 1.7 | 3.1 | 1.8 | < 10-8 |
| Creatine kinase-MB | ng/mL | 0.48 | 0.45 | 0.21 | 0.21 | 2.3 | 2.2 | 2.3 | 2.2 | 6.1 × 10-7 - 8.3 × 10-5 |
| C-reactive protein | ug/mL | 4.51 | 3.36 | 2.06 | 1.70 | 2.2 | 1.6 | 2.7 | 2.0 | 4.2 × 10-7 - 0.0074 |
| Myoglobin | ng/mL | 16.00 | 16.10 | 7.14 | 9.30 | 2.2 | 2.3 | 1.7 | 1.7 | < 10-8 - 3.0 × 10-8 |
| Stem cell factor | pg/mL | 395.00 | 402.00 | 221.00 | 191.00 | 1.8 | 1.8 | 2.1 | 2.1 | < 10-8 |
| IL-18 | pg/mL | 302.49 | 253.50 | 148.00 | 154.50 | 2.0 | 1.7 | 2.0 | 1.6 | < 10-8 |
| TNF-RII | pg/mL | 5.85 | 5.18 | 3.38 | 3.20 | 1.7 | 1.5 | 1.8 | 1.6 | < 10-8 |
| COPD < Control | ||||||||||
| IGF-1 | ng/mL | 45.30 | 29.30 | 188.55 | 203.00 | -4.2 | -6.4 | -4.5 | -6.9 | 3.0 × 10-7 - < 10-8 |
| IgE | ng/mL | 22.85 | 23.25 | 60.89 | 60.00 | -2.7 | -2.6 | -2.6 | -2.6 | 0.0043 - 6.0 × 10-6 |
aMedian serum concentrations of the analytes in the indicated population: T54, C0168T54 COPD; BS, BioServe COPD; Ctr1, healthy control population 1; Ctr2, healthy control population 2.
bSigned-fold = median in COPD/median in healthy control population. When median expression in COPD population was lower than in controls, the opposite of the reciprocal was reported.
cFalse discovery rate (calculated from Mann-Whitney p-values) for each of the 4 COPD vs control comparisons expressed as the range of FDR values.
dAnalytes significantly associated with COPD (FDR < 0.05 and |signed-fold| > 1.5) in each of the 4 comparisons are reported, ordered by descending average fold (COPD/control) values.
BDNF, brain-derived neurotrophic factor; COPD, chronic obstructive pulmonary disease; EGF, epidermal growth factor; ENA, epithelial-derived neutrophil activating protein; EN-RAGE, extracellular newly identified-receptor for advanced glycation end-binding protein; FDR, false discovery rate; IgE, immunoglobulin E; IGF, insulin-like growth factor; IL, interleukin; -MB, -muscle/brain; MCP, monocyte chemoattractant protein; MIP, macrophage inflammatory protein; PAI, plasminogen activating factor; RA, receptor agonist; RANTES, regulated upon activation, normally T-cell expressed, and secreted; TIMP, tissue inhibitor of metalloproteinases; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.