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Comparison of Alternative Assembly Methods
| Standard Assembly | Three Antibiotic Assembly | Amplified Insert Assembly | |
|---|---|---|---|
| Plasmid Extraction | X | X | X |
| Restriction Digest | X | X | X |
| Phosphatase Treatment | X | X | |
| DpnI Digest | X | ||
| Gel Electrophoresis | X | ||
| PCR | X | X | |
| Ligation | X | X | X |
| Purification | After Gel | Before Transformation | Before Digestion |
| Accuracy | Depends on size: Gel extraction difficult for small inserts | 89% | 99% |
