Table 3.
Parts and Plasmids
| Part# | Description | Size (bp) | Use | Plasmid | Resistance |
|---|---|---|---|---|---|
| BBa_I732018 | lacZ α with a strong ribosome binding site | 534 | Insert | pSB1AK3 | Ampicillin, Kanamycin |
| BBa_J23100 | Strong constitutive promoter in mRFP testing device | 35 + 890 | Insert | pBca1020 | Ampicillin |
| BBa_R0040 | TetR repressible promoter | 54 | Insert | pSB1A2 | Ampicillin |
| BBa_I732018 | lacZ α with a strong ribosome binding site | 534 | Vector | pSB1C3 | Chloramphenicol |
| BBa_J23100 | Strong constitutive promoter in mRFP testing device | 35 + 890 | Vector | pSB1C3 | Chloramphenicol |
The plasmids used are described according to the parts registry number www.partsregistry.org and the size of the BioBrick part contained on the plasmid. Parts given two sizes are promoters contained on promoter testing devices that have an mRFP coding sequence between the SpeI and PstI sites. The sizes are given as the promoter and mRFP gene respectively.