Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 9;302(3):C482–C493. doi: 10.1152/ajpcell.00140.2011

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2012 the American Physiological Society

PMC Copyright notice

Fig. 4. — Characterization of ANO7 antibody specificity. A: ANO7 antibody recognizes GFP-tagged ANO7 protein in transfected HEK293 cells and nontagged ANO7 in a stably transfected cell line 22Rv1-ANO7. No signal is detected in nontransfected HEK293 cells or 22Rv1 cells stably transfected with the empty vector. B: immunoblot showing that preincubation of ANO7 antibody with the immunizing peptide abolishes ANO7 staining in HEK293 cells transfected with GFP-tagged hANO7. C: ANO7 antibody (DAS1) staining in transfected HEK293 cells demonstrates that it colocalizes with myc-tagged ANO7. The staining is abolished upon preincubation of the antibody with immunizing peptide.