Fig. 4.

Hypoxia-induced activation of JNK. CMEC monolayers were exposed to control normoxic HEPES-DMEM at 19% O₂ or hypoxic medium at 7% O₂ or 2% O₂ for 5, 30, 60, or 120 min. Cell lysates were subjected to Western blot analysis using antibodies that recognize only phosphorylated (activated) JNK (p-JNK) or p-JNK and nonphosphorylated JNK (i.e., total JNK protein). A: representative Western blots. B: p-JNK abundance. Values are means ± SE of 6 and 5 separate experiments for 7% and 2% O₂, respectively. *Significantly different from control, P < 0.05 by 1-tailed paired t-test.