Fig. 6.

AVP-induced activation of JNK. Cells were exposed to control normoxic medium (glucose-containing HEPES-DMEM) or AVP (100 nM) in normoxic medium for 5, 30, 60, or 120 min. Cell lysates were subjected to Western blot analysis using antibodies that recognize JNK (total kinase) or p-JNK (phosphorylated, active kinase). A: representative Western blots. B: p-JNK abundance. Values are means ± SE for 6 separate experiments. *Significantly different from control, P < 0.05 by 1-tailed paired t-test.