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. 2011 Nov 2;302(3):C505–C517. doi: 10.1152/ajpcell.00261.2011

Fig. 7.

Fig. 7.

SB-239063 inhibition of hypoxia-stimulated CMEC Na-K-Cl cotransporter activity. CMEC monolayers were pretreated for 30 min with SB-239063 (20 μM) or vehicle and then exposed for 30–120 min to normoxic control (19% O2) or hypoxia at 7% O2 (A) or 2% O2 (B) in glucose-containing HEPES-DMEM also containing SB-239063 (20 μM) or vehicle. CMEC Na-K-Cl cotransporter activity was assessed as ouabain-insensitive, bumetanide-sensitive K influx. Pretreatment conditions were maintained throughout the assay. Values are means ± SE for 8 separate experiments. *Significantly different (vehicle vs. SB-239063), P < 0.05 by repeated-measures ANOVA with Tukey's post hoc test. Vehicle values are significantly different from control at 30, 60, 90, and 120 min (A) and at 30, 60, 90, and 120 min (B), P < 0.05 by 1-tailed paired t-test. For A and B, SB-23063 values in the presence of hypoxia are not significantly different from SB-239063 values of the normoxic control, by repeated-measures ANOVA.