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. 2011 Dec 2;40(4):e28. doi: 10.1093/nar/gkr1092

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The validation of AT5G16480 and Os10g08580 as targets of miR172 using the transient co-expression assay. N. benthamiana leaves were infiltrated with infiltration medium (mock); Agrobacteria harboring Ath-MIR172a alone (miR172); Agrobacteria harboring Arabidopsis transcript AT5G16480/rice transcript Os10g08580 alone (AT5G16480/Os10g08580); co-expression Ath-MIR172a and target genes (miR172+AT5G16480/miR172+Os10g08580). For the co-expression, equal amount of Agrobacterium culture containing Ath-MIR172a and AT5G16480 or Os10g08580 were mixed before infiltration into N. benthamiana leaves. U6 and actin are served as loading controls for miR172 and target gene (AT5G16480 or Os10g08580) detection, respectively. (a) The validation of AT5G16480. (b) The validation of Os10g08580.