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. 2011 Dec 10;40(4):e32. doi: 10.1093/nar/gkr1207

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Generation and characterization of a Bio-CxxC protein. (A) Schematic illustrating recombinant CxxC protein with N-terminal His tag (His-CxxC). Cleavage with TEV protease removes His tag to give CxxC alone. (B) EMSA demonstrating that His-CxxC and CxxC bind to a DNA probe containing non-methylated CpGs in a concentration-dependent manner (left panel), and that DNA methylation blocks this binding (right panel). (C) Schematic illustrating the generation of a CxxC protein with a C-terminal Avi-Tag (CxxC-Avi) and the subsequent reaction catalysed by the E. coli biotin ligase BirA to give a Bio-CxxC protein. (D) Mass spectrometry analysis of CxxC-Avi protein before (left panel) and after in vitro BirA reaction (right panel). Expected mass of CxxC-Avi is 18939 Da and Bio-CxxC is 19166 Da.