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Depletion of RECQL5 increases apoptosis and activates a G2/M checkpoint. (A) Dot plots of FITC-Annexin versus PI two parameter flow cytometry of HeLa shScrambled, shRECQL5-1 and shRECQL5-2 cells. (B) Quantification of Annexin positive and PI negative cells in HeLa. Error bars represent ±SD, n = 2; *P = 0.02, **P = 0.002, Student's t-test. (C and D) Western blot of whole cell lysates from HeLa shScrambled, shRECQL5-1 and shRECQL5-2 cells probed with indicated antibodies.
