Figure 6.
Assays of deoxyribozymes from the four selections that included the splint ligation pressure of Figure 5. Each DNA substrate had two unpaired nucleotides X^G, where X = one of C, T, A, or G, and is cleaved to form 3′-hydroxyl + 5′-phosphate termini. As observed for 13PB2 (Figure 2B), the single-turnover in trans catalytic activities were maintained for each deoxyribozyme when all nucleotides of the DNA substrate outside of the required cleavage-site X^G (Supplementary Figure S8) were changed systematically. See Supplementary Figure S7 for accompanying PAGE images. For 7VK55, C^G is tolerated at least as well as A^G (Supplementary Figure S9). Sequences of these deoxyribozymes are in Figure 3. kobs values (h−1) were as follows. (A) 7VH6: Par 0.20, Tv2 0.13, Tsn 0.095, Tv1 0.067. (B) 6YR25: Par 1.0, Tv2 0.43, Tsn 0.30, Tv1 0.18. (C) 7VK55: Par 0.094, Tv2 0.073, Tsn 0.13, Tv1 0.083. (D) 8LV51: Par 0.26, Tv2 0.16, Tsn 0.14, Tv1 0.22.