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. 2011 Oct 22;40(4):1460–1474. doi: 10.1093/nar/gkr865

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure. 4. — Suppression of the thoracic cleft induced by DREF knockdown in pnr-Gal4 heterozygous flies, by depletion of XNP/dATRX. (A) UAS-DREFi (top panel, left) or UAS-dATRXi (bottom panel, left) have a normal thorax, meanwhile flies with UAS-DREFi expression in the pnr domain driven by the pnr-Gal4 driver (middle and right top panels) show strong thoracic cleft defects. Depletion of XNP/dATRX in these flies, accomplished by the expression of the UAS-dATRXi transgene in the pnr domain, alleviates this defect (middle and right bottom panels). (B) pnr transcript levels are influenced by DREF and XNP/dATRX dosages. pnr-a andβ transcript levels in the thorax of flies depleted of DREF and DREF and XNP/dATRX in the pnr expression domain (genotypes as in Figure 4A). Note that in the thorax of DREF depleted flies there is a significant reduction of the pnr-β transcript levels. These levels are recovered when DREF and XNP/dATRX are simultaneously depleted in the pnr expression domain and partially recovered the ratio between pnr-α and pnr-β mRNAs. The quantification was obtained from three independent experiments and each cDNA was amplified three times by qRT–PCR. The Dmp8 transcript levels were used as reference control, which is not affected by the XNP/dATRX levels.