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1H jump-and return 2D-NOESY spectra (mixing time 200 ms) of the DNA duplexes containing (A) O:C, (B) O:T and (C) G:T mismatches collected at 5°C. The spectrum of the O:C duplex was measured at pH 7.0, and those of the O:T and G:T at pH 6.0. There was no significant difference in the chemical shift of the non-exchangeable protons at pH 6.0 and 7.0.
