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. 2011 Nov 2;40(4):1856–1867. doi: 10.1093/nar/gkr892

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effects of RNA mutations on RNA binding to BsHfq. (A) Mutant RNA sequences (bold type) constructed for UV cross-linking analysis. Tag sequence was added to 3′- and 5′-ends of RNA (italic) for in vitro transcription. AG repeat sequence is underlined. (B) UV cross-linking analysis of mutant RNA binding to BsHfq. ³²P-RNA (50 000 cpm) was incubated with 8.3 pmol of BsHfq-His and then loaded on gels. Lanes 1 and 5, BsHfq–His and 49R; lanes 2–4, BsHfq–His and mutants with GA(s) in AG repeat sequence replaced by CC(s) (49R-mt1, 49R-mt2 and 49R-mt3); lanes 6–9, BsHfq–His with mutants in which G in AG repeat sequence was replaced by U/A/C/GG (49R-U, 49R-A, 49R-C and 49R-GG).