Figure 6.

(a) Schematic representation of symmetric and asymmetric siRNA-expression cassettes, with the expression of either the sense or the antisense RNA or both being driven by a chimeric promoter(s) for disruption of their equivalent molar levels of production. (b) Western blotting analysis of knockdown of p53 expression in 293FT cells transduced with shRNA and with various siRNA-expression cassettes via lentivector pSD31. (c) Levels of expression of p53 mRNA in transduced 293FT cells, as determined by real-time RT-PCR. The data shown are relative to levels of p53 mRNA in pSD31-transduced 293FT cells. Data shown are derived from an experiment that was conducted in triplicate for each sample.