Skip to main content
. 2011 Nov 2;40(4):1695–1707. doi: 10.1093/nar/gkr934

Figure 4.

Figure 4.

miR-744 and miR-1186 promote phosphorylation of histone H3 and enhance in vitro cell proliferation. (A and B) NIH/3T3 and TRAMP C1 cells were transfected with 100 nM dsControl, miR-744 or miR-1186 for 5 days. Mock samples were transfected in the absence of miRNA. Total H3 and phosphorylated H3S10 (p-H3S10) levels were examined by immunoblot analysis using specific antibodies. (C and D) Total H3 and p-H3S10 levels were immunodetected in stable NIH/3T3 and TRAMP C1 sublines infected with either lenti-Ccnb1 or lenti-EV viral particles. (E) The doubling time of TRAMP C1 cells expressing empty vector (TRAMP-C1-miR-EV), miR-744 (TRAMP-C1-miR-744), miR-1186 (TRAMP-C1-miR-1186), or Ccnb1 (TRAMP-C1-Ccnb1) was evaluated over the course of 5 days. The average doubling time (hrs) ± SE of independent experiments is plotted in the corresponding bar graph; *P < 0.05, **P < 0.01, NS: not significant compared to the TRAMP C1 parental cell line. (F) TRAMP-C1-miR-EV or TRAMP-C1-miR-744 cells were transfected with 50 nM dsControl or siCcnb1. Cell proliferation was quantified at each day utilizing the CellTiter96 Aqueous One Solution. Error bars represents SE from four independent transfections. Statistical significance was determined between dsControl and siCcnb1 treatments within each subline; *P < 0.05, **P < 0.01.