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. 2012 Feb 13;122(3):1097–1108. doi: 10.1172/JCI46039

Figure 1. miR-21–ASO injected into the tail vein facilitates inhibition of increased miR-21 expression and derepression of its target genes after 2/3 PH.

Figure 1

(A) Time course of miR-21 expression after 2/3 PH. miR-21 levels were determined by qRT-PCR. Arrows indicate time points of miR-21–ASO or carrier injection and liver analysis relative to 2/3 PH. Liver samples obtained by 2/3 PH (Analysis 0 hours after 2/3 PH) were used to define baseline levels in the quiescent liver. (B) qRT-PCR showed that miR-21–ASO injections at 6 or 10 hours after 2/3 PH were similarly effective in antagonizing the peak of the surge in miR-21 expression after 2/3 PH. (C) Repression of Btg2 mRNA levels in livers of control mice after 2/3 PH was reversed in mice injected with miR-21–ASO at 6 or 10 hours after 2/3 PH. At least 3 mice were analyzed for each time point and treatment. Control mice were injected with carrier. Data represent mean ± SEM. *P < 0.05.