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. 2012 Feb 6;122(3):1037–1051. doi: 10.1172/JCI59110

Figure 6. Anti-GD1a Ab uptake protects NMJs from injury in vivo.

Figure 6

(A) Sternomastoid muscles from live anesthetized mice were labeled with anti-GD1a together with NHS (0 minutes) or anti-GD1a alone for 30 minutes, followed by 60 minutes with Ringer’s before exposure to NHS. Control mice received Ringer’s alone. NMJs were visually assessed for the loss of CFP overlying the acetylcholine receptor staining, and the percentage of NMJs that retained the CFP (healthy NMJs) was calculated. There is an increase in healthy NMJs in tissue incubated at 37°C. NMJs incubated in Ringer’s for 60 minutes (to allow Ab uptake) before exposure to NHS are protected from injury. Scale bar: 50 μm. (B) Quantitative analysis of NMJ integrity showing significant preservation of the endplate structure (arrows in A) in tissues subjected to a gap of 60 minutes incubation with Ringer’s prior to exposure to NHS. n = 3; **P < 0.001.