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. 2012 Feb 6;122(3):1022–1036. doi: 10.1172/JCI59701

Figure 11. BNP infusion increased oxygen consumption, energy expenditure, and expression of brown adipocyte markers.

Figure 11

C57BL/6 mice were treated for 7 days with saline (0.9% NaCl) or with BNP (2 ng/kg/ml) via Mini-pump. Food intake, physical activity, and respiration were measured by the Oxymax Comprehensive Lab Animal Monitoring System. (A) Plasma BNP levels measured on day 7. (B) Oxygen consumption and (C) energy expenditure (EE) during the light and dark periods. *P < 0.05; **P < 0.01; ***P < 0.001. Western blot in (D) iBAT (BAT) and (E) inguinal WAT for UCP1, PGC-1α, and cytochrome c protein levels. GAPDH was used as internal standard.