Figure 9. Combining β-agonist and ANP treatment augments their individual capacities to induce brown adipocyte markers.

hMADS adipocytes were treated with (A–C) 1 nM or (D–F) 10 nM of ANP or Iso, both alone and together (ANP+Iso), for 6 hours, and mRNA levels were measured for (A and D) UCP1, (B and E) PGC-1α, and (C and F) CYCS. *P < 0.05, **P < 0.01, ***P < 0.001 versus untreated cells; ^#P < 0.01 for ANP plus Iso versus ANP or Iso treatment alone. (G) In a sample experiment, similarly treated hMADS adipocytes were harvested, cell lysates were prepared, and Western blotting using specific antisera was performed, as indicated in Methods, and normalized for GAPDH (values are reported under each band.