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. 2012 Feb 13;122(3):974–986. doi: 10.1172/JCI60588

Figure 6. Characterization of IL-17A–producing cells in the lung, gut, and spleen of WT, Cxcl5–/–, and Cxcr2–/– mice.

Figure 6

(A) Representative flow cytometry histograms of isolated lung cells, LP cells from terminal ileum, and splenocytes from WT, Cxcl5–/–, and Cxcr2–/– mice (n = 3 mice/group). These cells were immunostained for intracellular IL-17A and surface CD3 expression. Representative histograms with immunostaining for surface CD4, TCRβ, TCRγδ, Gr-1, NK1.1 and intracellular IL-17A are shown in Supplemental Figures 1–3. These histograms are the representative of 2 separate experiments with similar results. (B) IL-17A–producing cell percentages of different cell populations in the lung, LP of terminal ileum, and spleen. Values are means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.