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Proliferative kinetics of AIV H5N1 in BHK21 cells. BHK21 cells were infected directly with H5N1 AIV, and the supernatant and bottom cells of the sample at 12, 24, 36, 48, 60, 72 h were collected respectively. Finally, samples separated were examined by Western blotting with anti-M1 antibody respectively, with β-actin as internal control. The result showed that the volume of the M1 protein had significant growth at 48 h, and less obvious growth afterward.
