. Author manuscript; available in PMC: 2012 Feb 27.

Published in final edited form as: J Pathol. 2010 Oct;222(2):138–147. doi: 10.1002/path.2746

Table 2. RT-PCR and qPCR primer sequences and cycling conditions.

Cycling conditions comprised an initial 10 min of polymerase activation at 95°C and a final extension of 10 min at 72°C.

RT-PCR	SENSE AND ANTISENSE SEQUENCES	PCR CONDITIONS
VEGF_xxx/VEGF_xxxb		35 Cycles:
	S: 5′-GTTTGTACAAGATCCGCAGACGT-3′	95°C for 15s
	A: 5′-ATGGATCCGTATCAGTCTTTCCTGG-3′	56°C for 30s
		60°C for 30s

qPCR	SENSE AND ANTISENSE SEQUENCES	PCR CONDITIONS

VEGF_xxx	S: 5′-GTTTGTACAAGATCCGCAGACGT-3′	45 Cycles:
	A: 5′-GTTCCCGAAACCCTGAGGG-3′	95°C for 15s
		60°C for 60s
SRPK1	Validated primers (Qiagen, QT00059556)	45 Cycles:
		95°C for 15s
		55°C for 30s
		72°C for 30s
β-Actin	S: 5′- AACGGGAAGCTCACTGGCATG-3′	45 Cycles:
	A: 5′- TCCACCACCCTGTTGCTGTAG-3′	95°C for 15s
		60°C for 60s
HRPT I	S: 5′-TGACACTGGCAAAACAATGCA-3′	45 Cycles:
	A: 5′-GGTCCTTTTCACCAGCAAGCT-3′	95°C for 15s
		60°C for 60s
SDHA	S: 5′-TGGGAACAAGAGGGCATCTG-3′	45 Cycles:
	A: 5′-CCACCACTGCATCAAATTCATG-3′	95°C for 15s
		60°C for 60s